Quantification of Chemical Components and Phytochemical Screening of GaliumVerum by HPLC and LC-MS Analysis

Abstract

Galium verum L. – yellow "Lady's bedstraw" – contains polyphenols, flavonoids, phenyl- propanoids compounds, and iridoids in its golden yellow blossoms. The chemical makeup of the volatile fraction of Galiumverum L. was analysed.This plant is from the natural flora of Moldova (RediuIasi region, Romania) as a source of polyphenols and flavonoids.The purpose of this investigation is to investigate the composition of the herb methanol extract of Lady's bedstraw (Galium verum L.) using liquid chromatography with mass spectrometric(LCMS) detection and the HPLC method.Herb of G. verum L. that was air-dried was used to prepare methanolicextract. The extract is then employed for HPLC and LC-MS analysis. It was determined that the methanolic extract of Lady's bedstraw is abundant in biologically active substances that determine its broad spectrum of pharmacological action.By comparing certain compounds' mass spectra to libraries, several compounds were identified.

Keywords: HPLC;LC-MS;UV activecompounds; Quantification; GaliumVerum; Phytochemical
Screening,