Open Access Open Access  Restricted Access Subscription or Fee Access

Studies on Evaluation and Production of Cordyceps Militaris Potency: In Vivo on Drosophila Melanogaster

Sandeep Sirohi, Adarsh Singh, Raghav Singh

Abstract


This research looked at the impact of Cordyceps militaris oral liquid (CSOL) on the lifespan and metabolic activities of Drosophila melanogaster (fruit fly). The longevity of fruit flies after a lifetime of CMOL treatment was investigated. Activity of the superoxide dismutase 1 (SOD1), Supro-oxide dismutase 2 (SOD2) and Catalase (CAT) containing copper-zinc were evaluated as well as concentrations of lipofuscin (LF). SOD1, SOD2, and CAT mRNA were utilized in the measurement of qPCR. To simulate the consequences of acute oxidative stress, researchers employed hydrogen peroxide (H2O2) and paraquat. Chronic pathological ageing was simulated using D-galactose. Under physiological circumstances, CMOL substantially increased the fruit fly's lifetime. The SOD1 and CAT activities have been uncontrolled and LF accumulation has been avoided by CMOL. CMOL was not influenced by the transcriptional level of these enzymes (mRNA). The period of survival of flies affected by H2O2 or paraquat exposure has been significantly enhanced by CMOL. The durability of Fruit Flies, aged with D-galactose exposure, regulating SOD1 and CAT, and preventing LF buildup in D-galactose-exposed Flies has been considerably increased by CMOL. Our findings show that CMOL prolong the fruit flies life through an anti-oxidative stress mechanism with enhanced SOD1 and CAT activity as well as the removal of LF accumulation. As a result, CMOL might be investigated as a new agent for delaying the ageing process in humans.


Keywords


Cordyceps militaris, Aging, Lifespan, Oxidative stress, Drosophila melanogaster

Full Text:

PDF

References


Zou Y, Liu Y, Ruan M, Feng X, Wang J, Chu Z, Zhang Z. Cordyceps sinensis oral liquid prolongs the lifespan of the fruit fly, Drosophila melanogaster, by inhibiting oxidative stress. Int J Mol Med. 2015 Oct; 36(4):939-46.

Zou YX, Ruan MH, Luan J, Feng X, Chen S, Chu ZY. Anti-Aging Effect of Riboflavin via Endogenous Antioxidant in Fruit fly Drosophila Melanogaster. J Nutr Health Aging. 2017; 21(3):314-319.

Fleming JE, Reveillaud I, Niedzwiecki A. Role of oxidative stress in Drosophila aging. Mutat Res. 1992 Sep; 275(3-6): 267-79.

Peng C, Chan HY, Li YM, Huang Y, Chen ZY. Black tea theaflavins extend the lifespan of fruit flies. Exp Gerontol. 2009 Dec; 44(12): 773-83

Zuo Y, Peng C, Liang Y, Ma KY, Yu H, Edwin Chan HY, Chen ZY. Black rice extract extends the lifespan of fruit flies. Food Funct. 2012 Dec; 3(12): 1271-9

Peng C, Chan HY, Li YM, Huang Y, Chen ZY. Black tea thea flavins extend the lifespan of fruit flies. Exp Gerontol. 2009 Dec; 44(12): 773-83

Cui X, Wang L, Zuo P, Han Z, Fang Z, Li W, Liu J. D-galactose-caused life shortening in Drosophila melanogaster and Musca domestica is associated with oxidative stress. Biogerontology. 2004; 5(5): 317-25.

Huangfu J, Liu J, Sun Z, Wang M, Jiang Y, Chen ZY, Chen F. Antiaging effects of astaxanthin-rich alga Haematococcus pluvialis on fruit flies under oxidative stress. J Agric Food Chem. 2013 Aug 14; 61(32):7800-4.

Zuo Y, Peng C, Liang Y, Ma KY, Chan HY, Huang Y, Chen ZY. Sesamin extends the mean lifespan of fruit flies. Biogerontology. 2013 Apr; 14(2): 107-19.

Chattopadhyay D, Chitnis A, Talekar A, Mulay P, Makkar M, James J, Thirumurugan K. Hormetic efficacy of rutin to promote longevity in Drosophila melanogaster. Biogerontology. 2017 Jun; 18(3):3 97-411.

Li YM, Chan HY, Huang Y, Chen ZY. Green tea catechins upregulate superoxide dismutase and catalase in fruit flies. Mol Nutr Food Res. 2007 May; 51(5): 546-54.

Leow SS, Luu A, Shrestha S, Hayes KC, Sambanthamurthi R. Drosophila larvae fed palm fruit juice (PFJ) delay pupation via expression regulation of hormetic stress response genes linked to ageing and longevity. Exp Gerontol. 2018 Jun; 106: 198-221.

Sun Y, Yolitz J, Alberico T, Sun X, Zou S. Lifespan extension by cranberry supplementation partially requires SOD2 and is life stage independent. Exp Gerontol. 2014 Feb; 50: 57-63.

Lushchak OV, Gospodaryov DV, Yurkevych IS, Storey KB. Oxidized Lipids did not Reduce Lifespan in the Fruit Fly, Drosophila melanogaster. Arch Insect Biochem Physiol. 2016 Jan; 91(1): 52-63.

Shchedrina VA, Vorbrüggen G, Lee BC, Kim HY, Kabil H, Harshman LG, Gladyshev VN. Overexpression of methionine-R-sulfoxide reductases has no influence on fruit fly aging. Mech Ageing Dev. 2009 Jul; 130(7): 429-43.

Zou YX, Liu YX, Ruan MH, Zhou Y, Wang JC, Chu ZY. Cordyceps sinensis Oral Liquid Inhibits Damage Induced by Oxygen and Glucose Deprivation in SH-SY5Y Cells. Altern Ther Health Med. 2016 Mar-Apr; 22(2): 37-42

Wang HL, Sun ZO, Rehman RU, Wang H, Wang YF, Wang H. Rosemary Extract-Mediated Lifespan Extension and Attenuated Oxidative Damage in Drosophila melanogaster Fed on High-Fat Diet. J Food Sci. 2017 Apr; 82(4): 1006-1011.

Chen S, Yang Q, Chen X, Tian Y, Liu Z, Wang S. Bioactive peptides derived from crimson snapper and in vivo anti-aging effects on fat diet-induced high fat Drosophila melanogaster. Food Funct. 2020 Jan 29; 11(1): 524-533

Tsuda M, Ootaka R, Ohkura C, Kishita Y, Seong KH, Matsuo T, Aigaki T. Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila. FEBS Lett. 2010 Aug 4; 584(15): 3398-401.

Ruan Q, Qiao Y, Zhao Y, Xu Y, Wang M, Duan J, Wang D. Beneficial effects of Glycyrrhizae radix extract in preventing oxidative damage and extending the lifespan of Caenorhabditis elegans. J Ethnopharmacol. 2016 Jan 11; 177: 101-10.

Li X, Zhang Z, Zhang X, Cheng J, Liu D, Yan Y, Wang H. Transcriptomic analysis of the life-extending effect exerted by black rice anthocyanin extract in D. melanogaster through regulation of aging pathways. Exp Gerontol. 2019 May; 119: 33-39.

Lashmanova E, Proshkina E, Zhikrivetskaya S, Shevchenko O, Marusich E, Leonov S, Melerzanov A, Zhavoronkov A, Moskalev A. Fucoxanthin increases lifespan of Drosophila melanogaster and Caenorhabditis elegans. Pharmacol Res. 2015 Oct; 100: 228-41.

Sun X, Komatsu T, Lim J, Laslo M, Yolitz J, Wang C, Poirier L, Alberico T, Zou S. Nutrient-dependent requirement for SOD1 in lifespan extension by protein restriction in Drosophila melanogaster. Aging Cell. 2012 Oct; 11(5): 783-93.

Lashmanova EA, Kuzivanova OA, Dymova OV, Moskalev AA. [The effects of cloudberry extract on Drosophila melanogaster lifespan and stress resistance. Adv Gerontol. 2018; 31(6): 958-965.

Trindade de Paula M, Poetini Silva MR, Machado Araujo S, Cardoso Bortolotto V, Barreto Meichtry L, Zemolin AP, Wallau GL, Jesse CR, Franco JL, Posser T, Prigol M. High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster. Oxid Med Cell Longev. 2016; 2016: 4018157.

Figueira, F.H., de Aguiar, L.M. and da Rosa, C.E., 2017. Embryo-larval exposure to atrazine reduces viability and alters oxidative stress parameters in Drosophila melanogaster. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 191, pp.78-85.


Refbacks

  • There are currently no refbacks.